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Antibodies

Ube2L3 / UBCH7 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A20887-20UL
100 ul
£342.00
A20887-100UL
About this Product
SKU:
A20887
Additional Names:
E2-F1|L-UBC|UBCH7|UbcM4|Ube2L3 / UBCH7
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
18kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
INFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A20887
Western blot analysis of various lysates, using Ube2L3 / UBCH7 Rabbit mAb (A20887) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of K-562 cells using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.