Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

HNF-4-alpha Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A20865-20UL
100 ul
£342.00
A20865-100UL
500 ul
£1532.00
A20865-500UL
1000 ul
£2704.00
A20865-1000UL
About this Product
SKU:
A20865
Additional Names:
HNF-4|HNF-4-alpha|Hnf4|Hnf4alpha|MODY1|Nr2a1|TCF-14|Tcf14
Application:
ChIP, ELISA, IHC-Paraffin, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
53kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MRLSKTLAGMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKKIANITDVCESMKEQLLVLVE
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A20865
Western blot analysis of various lysates using HNF-4-alpha Rabbit mAb (A20865) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using HNF-4-alpha Rabbit mAb (A20865) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using HNF-4-alpha Rabbit mAb (A20865) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human small intestine using HNF-4-alpha Rabbit mAb (A20865) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using HNF-4-alpha antibody (A20865) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.