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Antibodies

Ret Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A20845-20UL
100 ul
£342.00
A20845-100UL
About this Product
SKU:
A20845
Additional Names:
CDHF12|CDHR16|HSCR1|MEN2A|MEN2B|MTC1|PTC|Ret|RET-ELE1
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
124 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
DISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRALPSTWIENKLYGMSDPNWPGESPVPLTRADGTNTGFPRYPNDSVYANWM
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A20845
Western blot analysis of lysates from TT cells using Ret Rabbit mAb (A20845) at 1:2000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20 s.
Western blot analysis of lysates from Neuro-2a cells using Ret Rabbit mAb (A20845) at 1:2000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20 s.
Confocal imaging of THP-1 cells using Ret Rabbit mAb (A20845, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red). DAPI was used for nuclear staining (Blue). Objective: 100x.