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Antibodies

SARS-CoV-2 N Protein Mouse monoclonal antibody

Product Sizes
20 ul
£164.00
A20142-20UL
100 ug
£342.00
A20142-100UG
About this Product
SKU:
A20142
Additional Names:
NP|Nucleoprotein|SARS-CoV-2 N Protein
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG1, kappa
Molecular Weight:
46kda
Purification:
Affinity Purified
Reactivities:
Virus
Sequence:
MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADST
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A20142
Western blot analysis of from wild type (WT) and 293T cells transfected with SARS-CoV-2 N Protein using SARS-CoV-2 N Protein Mouse mAb (A20142) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 20ug/10ug/5ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Immunofluorescence analysis of 293T transfected with SARS-CoV-2 N Protein using SARS-CoV-2 Nucleoprotein mAb mAb (A20142) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immobilized Recombinant 2019-nCoV Nucleocapsid Protein (RP01264LQ) at 1ug/mL (100uL/well) can bind SARS-CoV-2 Nucleoprotein Rabbit mAb (A20142) with a linear range of 3.12-200ng/mL.