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Antibodies

SARS-CoV-2 Spike S2 ECD Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A20138-20UL
100 ul
£342.00
A20138-100UL
About this Product
SKU:
A20138
Additional Names:
SARS-CoV-2 Spike S2 ECD|spike glycoprotein
Application:
Dot Blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
141kDa
Purification:
Affinity Purified
Reactivities:
Human, Virus
Sequence:
SVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPW
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A20138
Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD Rabbit pAb (A20138) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s.
Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein(RP01283LQ) at 1ug/mL (100uL/well) can bind SARS-CoV-2 Spike S2 ECD Rabbit pAb (A20138) with a linear range of 3.12-50ng/mL.
Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S2 ECD fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 Spike S2 ECD Rabbit pAb (A20138) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug SARS-CoV-2 Spike S2 ECD antibody (A20138). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 ECD antibody (A20138) at a dilution of 1:5000.