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Antibodies

[KO Validated] B Beta-Catenin Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A19657-20UL
100 ul
£342.00
A19657-100UL
500 ul
£1532.00
A19657-500UL
1000 ul
£2704.00
A19657-1000UL
About this Product
SKU:
A19657
Additional Names:
armadillo|CTNNB|EVR7|in|MRD19|NEDSDV
Application:
ChIP, ELISA, IHC-Paraffin, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
85kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A19657
Western blot analysis of various lysates using [KO Validated] B Beta-Catenin Rabbit mAb (A19657) at 1:4000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Western blot analysis of lysates from HeLa cells using [KO Validated] B Beta-Catenin Rabbit mAb (A19657) at 1:4000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
Western blot analysis of lysates from wild type (WT) and B Beta-Catenin knockout (KO) 293T cells using [KO Validated] B Beta-Catenin Rabbit mAb (A19657) at 1:4000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using [KO Validated] B Beta-Catenin Rabbit mAb (A19657) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] B Beta-Catenin Rabbit mAb (A19657) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.