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Antibodies

SERCA1/ATP2A1 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A19639-20UL
100 ul
£342.00
A19639-100UL
About this Product
SKU:
A19639
Additional Names:
ATP2A|SERCA1|SERCA1/ATP2A1
Application:
ELISA, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
95kDa/110kDa
Purification:
Affinity Purified
Reactivities:
Mouse, Rat
Sequence:
ALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEDPEDERRK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A19639
Western blot analysis of lysates from Mouse skeletal muscle, using SERCA1/ATP2A1 Rabbit mAb (A19639) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Confocal imaging of paraffin-embedded Mouse skeletal muscle tissue using SERCA1/ATP2A1 Rabbit mAb (A19639, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Rat skeletal muscle tissue using SERCA1/ATP2A1 Rabbit mAb (A19639, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.