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Antibodies

Cleaved PARP1 p25 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A19612-20UL
100 ul
£342.00
A19612-100UL
500 ul
£1532.00
A19612-500UL
1000 ul
£2704.00
A19612-1000UL
About this Product
SKU:
A19612
Additional Names:
ADPRT|ADPRT 1|ADPRT1|ARTD1|Cleaved PARP1 p25|pADPRT-1|PARP|PARP-1|PARS|Poly-PARP|PPOL
Application:
ELISA, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-214 of human Cleaved PARP1 p25 (P09874).
Isotype:
IgG
Molecular Weight:
113 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
QLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVD
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A19612
Western blot analysis of lysates from wild type (WT) and Cleaved PARP1 p25 knockout (KO) cells using Cleaved PARP1 p25 Rabbit mAb (A19612) at 1:10000 dilution incubated at room temperature for 1.5 hours. Wild type (WT) and PARP1 knockout (KO) HeLa cells were treated with Staurosporine(1 uM) at 37°C for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Western blot analysis of lysates from Jurkat cells using Cleaved PARP1 p25 Rabbit mAb (A19612) at 1:10000 dilution incubated at room temperature for 1.5 hours. Jurkat cells were treated with Etoposide (25 uM) at 37°C for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.