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Antibodies

[KD Validated] TRAF2 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A19129-20UL
100 ul
£342.00
A19129-100UL
500 ul
£1532.00
A19129-500UL
1000 ul
£2704.00
A19129-1000UL
About this Product
SKU:
A19129
Additional Names:
[KD Validated] TRAF2|MGC:45012|RNF117|TRAP|TRAP3
Application:
ELISA, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
53kDa/54kDa/55kDa/61kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
DGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTY
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A19129
Western blot analysis of various lysates, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of various lysates, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Western blot analysis of lysates from wild type(WT) and TRAF2 knockdown (KD) 293T cells, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug TRAF2 antibody (A19129). Western blot was performed from the immunoprecipitate using TRAF2 antibody (A19129) at a dilution of 1:50000.