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Antibodies

[KO Validated] Smad1 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A19113-20UL
100 ul
£342.00
A19113-100UL
500 ul
£1532.00
A19113-500UL
1000 ul
£2704.00
A19113-1000UL
About this Product
SKU:
A19113
Additional Names:
BSP-1|BSP1|d1|JV4-1|JV41|MADH1|MADR1
Application:
ELISA, IHC-Paraffin, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
52kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
WKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQ
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A19113
Western blot analysis of lysates from Mouse brain, using Smad1 Rabbit mAb (A19113) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Western blot analysis of lysates from wild type(WT) and Smad1 Rabbit mAb knockout (KO) HeLa(KO) cells, using Smad1 Rabbit mAb (A19113) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using [KO Validated] Smad1 Rabbit mAb (A19113) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation of Smad1 from 400 ug extracts of HeLa cells was performed using 2 ug of [KO Validated] Smad1 Rabbit mAb (A19113). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KO Validated] Smad1 Rabbit mAb (A19113) at a dilution of 1:500.