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Antibodies

[KO Validated] N-Cadherin Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A19083-20UL
100 ul
£342.00
A19083-100UL
500 ul
£1532.00
A19083-500UL
1000 ul
£2704.00
A19083-1000UL
About this Product
SKU:
A19083
Additional Names:
ACOGS|ADHD8|ARVD14|CD325|CDHN|CDw325|in|NCAD
Application:
ELISA, IHC-Paraffin, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
100 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
AKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGP
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A19083
Western blot analysis of lysates from wild type (WT) and N-Cadherin knockout (KO) HeLa cells using [KO Validated] N-Cadherin Rabbit mAb (A19083) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.
Western blot analysis of lysates from C2C12 cells using [KO Validated] N-Cadherin Rabbit mAb (A19083) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Western blot analysis of lysates from C6 cells using [KO Validated] N-Cadherin Rabbit mAb (A19083) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] N-Cadherin Rabbit mAb (A19083) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] N-Cadherin Rabbit mAb (A19083) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.