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Antibodies

GAPDH Rabbit monoclonal antibody(High Dilution)

Product Sizes
50 ul
£158.00
A19056-50UL
100 ul
£235.00
A19056-100UL
200 ul
£366.00
A19056-200UL
500 ul
£747.00
A19056-500UL
1000 ul
£1348.00
A19056-1000UL
About this Product
SKU:
A19056
Additional Names:
G3PD|GAPD|GAPDH|HEL-S-162eP
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
36kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
VKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A19056
Western blot analysis of lysates from HeLa cells, using GAPDH Rabbit mAb (A19056) at 1:10000-1:2560000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of various lysates using GAPDH Rabbit mAb(High Dilution) (A19056) at 1:900000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30 s.
Immunohistochemistry analysis of paraffin-embedded Human colon using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human spleen using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.