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Antibodies

Cytokeratin 19 (CK19) Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A19040-20UL
100 ul
£342.00
A19040-100UL
500 ul
£1532.00
A19040-500UL
1000 ul
£2704.00
A19040-1000UL
About this Product
SKU:
A19040
Additional Names:
Cytokeratin 19 (CK19)|K19|K1CS|KRT19
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
44 kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
MTSYSYRQSSATSSFGGLGGGSVRFGPGVAFRAPSIHGGSGGRGVSVSSARFVSSSSSGAYGGGYGGVLTASDGLLAGNEKLTMQNLNDRLASYLDKVRA
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A19040
Western blot analysis of various lysates using Cytokeratin 19 (CK19) Rabbit mAb (A19040) at 1:500 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): HeLa Exposure time: 0.5s.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Cytokeratin 19 (CK19) Rabbit mAb (A19040) at a dilution of 1:2500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Cytokeratin 19 (CK19) Rabbit mAb (A19040) at a dilution of 1:2500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Cytokeratin 19 (CK19) Rabbit mAb (A19040) at a dilution of 1:2500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of MCF7 cells using Cytokeratin 19 (CK19) Rabbit mAb (A19040, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.