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Antibodies

H2-Ab1 Rabbit polyclonal antibody

Product Sizes
20 ul
£152.00
A18658-20UL
100 ul
£336.00
A18658-100UL
500 ul
£1258.00
A18658-500UL
1000 ul
£2228.00
A18658-1000UL
About this Product
SKU:
A18658
Additional Names:
Abeta|H-2Ab|H2-Ab|H2-Ab1|I-Abeta|Ia-2|Ia2|IAb|Rmcs1
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
30kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
SERHFVYQFMGECYFTNGTQRIRYVTRYIYNREEYVRYDSDVGEHRAVTELGRPDAEYWNSQPEILERTRAELDTVCRHNYEGPETHTSLRRLEQPNVVISLSRTEALNHHNTLVCSVTDFYPAKIKVRWFRNGQEETVGVSSTQLIRNGDWTFQVLVMLEMTPRRGEVYTCHVEHPSLKSPITVEWRAQ
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A18658
Western blot analysis of lysates from Mouse large intestine, using H2-Ab1 Rabbit pAb (A18658) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using H2-Ab1 Rabbit pAb (A18658) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH-3T3 cells using H2-Ab1 Rabbit pAb (A18658) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.