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Antibodies

[KO Validated] PTBP1 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A1831-20UL
100 ul
£342.00
A1831-100UL
About this Product
SKU:
A1831
Additional Names:
HNRNP-I|HNRNPI|HNRPI|P1|pPTB|PTB|PTB-1|PTB-T|PTB2|PTB3|PTB4
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
60kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDSKKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYTRPDLPSGDSQPSLD
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A1831
Western blot analysis of lysates from wild type (WT) and PTBP1 knockout (KO) HeLa cells, using [KO Validated] PTBP1 Rabbit pAb (A1831) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Western blot analysis of various lysates, using [KO Validated] PTBP1 Rabbit pAb (A1831) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Western blot analysis of lysates from wild type(WT) and PTBP1 knockout (KO) HeLa(KO) cells, using [KO Validated] PTBP1 Rabbit pAb (A1831) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] PTBP1 Rabbit pAb (A1831) at dilution of 1:25 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal immunofluorescence analysis of U-2OS cells using [KO Validated] PTBP1 Rabbit pAb (A1831) at dilution of 1:50. Blue: DAPI for nuclear staining.