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Antibodies

Acetyl-Histone H3-K4 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A16078-20UL
100 ul
£342.00
A16078-100UL
About this Product
SKU:
A16078
Additional Names:
Acetyl-Histone H3-K4|H3.4|H3/g|H3C16|H3FT|H3t|HIST3H3
Application:
ChIP, ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
15 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Other Species, Rat
Sequence:
ARTKQ
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A16078
Western blot analysis of lysates from NIH/3T3 cells using Acetyl-Histone H3-K4 Rabbit pAb (A16078) at 1:400 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Western blot analysis of lysates from C6 cells using Acetyl-Histone H3-K4 Rabbit pAb (A16078) at 1:400 dilution. C6 cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Western blot analysis of lysates from HeLa cells using Acetyl-Histone H3-K4 Rabbit pAb (A16078) at 1:400 dilution. HeLa cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K4 Rabbit pAb (A16078) at dilution of 1:100.C6 cells were treated with TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K4 Rabbit pAb (A16078) at dilution of 1:100.HeLa cells were treated with TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.