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Antibodies

Acetyl-Histone H4-K5 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A15233-20UL
100 ul
£342.00
A15233-100UL
About this Product
SKU:
A15233
Additional Names:
Acetyl-Histone H4-K5|FO108|H4|H4-16|H4/n|H4C1|H4C11|H4C12|H4C13|H4C15|H4C16|H4C2|H4C3|H4C4|H4C5|H4C6|H4C8|H4C9|H4F2|H4FN|HIST2H4|HIST2H4A
Application:
ChIP, ELISA, IHC-Paraffin, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
11kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Other Species, Rat
Sequence:
MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYG
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A15233
Western blot analysis of various lysates using Acetyl-Histone H4-K5 Rabbit pAb (A15233) at 1:1000 dilution. HeLa cells were treated with TSA (1uM) for 18 hours. C6 cells were treated with TSA (1uM) for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human lung using Acetyl-Histone H4-K5 Rabbit pAb (A15233) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Acetyl-Histone H4-K5 Rabbit pAb (A15233) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using Acetyl-Histone H4-K5 Rabbit pAb (A15233) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 Rabbit pAb antibody (A15233) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.