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Antibodies

GLI1 Rabbit polyclonal antibody

Product Sizes
20 ul
£152.00
A14675-20UL
100 ul
£336.00
A14675-100UL
500 ul
£1258.00
A14675-500UL
1000 ul
£2228.00
A14675-1000UL
About this Product
SKU:
A14675
Additional Names:
GLI|GLI1|PAPA8|PPD1
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
118kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
LSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A14675
Western blot analysis of lysates from Mouse brain, using GLI1 Rabbit pAb (A14675) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using GLI1 Rabbit pAb (A14675) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse uterus using GLI1 Rabbit pAb (A14675) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using GLI1 Rabbit pAb (A14675) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of A-549 cells using GLI1 Rabbit pAb (A14675) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.