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Antibodies

[KO Validated] Glutathione Synthetase (GSS) Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A14535-20UL
100 ul
£342.00
A14535-100UL
About this Product
SKU:
A14535
Additional Names:
GSHS|HEL-S-64p|HEL-S-88n|S)
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
52kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
NLYGEEMVQALKQLKDSEERASYILMEKIEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C 50% glycerol. Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A14535
Western blot analysis of lysates from wild type (WT) and Glutathione Synthetase (Glutathione Synthetase (GSS)) knockout (KO) 293T cells, using [KO Validated] Glutathione Synthetase (GSS) Rabbit pAb (A14535) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Immunofluorescence analysis of NIH/3T3 cells using Glutathione Synthetase (GSS) Rabbit pAb (A14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Glutathione Synthetase (GSS) Rabbit pAb (A14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Glutathione Synthetase (GSS) Rabbit pAb (A14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.