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Antibodies

[KO Validated] PMS2 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A13680-20UL
100 ul
£342.00
A13680-100UL
About this Product
SKU:
A13680
Additional Names:
HNPCC4|LYNCH4|MLH4|MMRCS4|PMS-2|PMS2CL|PMSL2|S2
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
96kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
ADLEKPMVEKQDQSPSLRTGEEKKDVSISRLREAFSLRHTTENKPHSPKTPEPRRSPLGQKRGMLSSSTSGAISDKGVLRPQKEAVSSSHGPSDPTDRAEVEKDSGHGSTSVDSEGFSIPDTGSHCSSEYAASSPGDRGSQEHVDSQEKAPKTDDSFSDVDCHSNQEDTGCKFRVLPQPTNLATPNTKRFKKEEILSSSDICQKLVNTQDMSASQVDVAVKINKKVVPLDFSMSSLAKRIKQLHHEAQQSEGEQNYRKFRAKICPGENQAAEDELRKEISK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A13680
Western blot analysis of various lysates using [KO Validated] PMS2 Rabbit pAb (A13680) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Western blot analysis of lysates from wild type(WT) and PMS2 knockout (KO) HeLa(KO) cells, using [KO Validated] PMS2 Rabbit pAb (A13680) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using [KO Validated] PMS2 Rabbit pAb (A13680).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.
Immunoprecipitation analysis of 150 ug extracts of Jurkat cells using 3 ug PMS2 antibody (A13680). Western blot was performed from the immunoprecipitate using PMS2 antibody (A13680) at a dilution of 1:500.