Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

[KO Validated] BAF57/SMARCE1 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A13353-20UL
100 ul
£342.00
A13353-100UL
About this Product
SKU:
A13353
Additional Names:
BAF57|CSS5|E1
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
47kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYLAYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEVDMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEKKE
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A13353
Western blot analysis of various lysates using [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and BAF57/SMARCE1 knockout (KO) 293T cells, using [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunofluorescence analysis of HeLa cells using [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.
Immunoprecipitation analysis of 150 ug extracts of Jurkat cells using 3 ug BAF57/BAF57/SMARCE1 antibody (A13353). Western blot was performed from the immunoprecipitate using BAF57/BAF57/SMARCE1 antibody (A13353) at a dilution of 1:1000.