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Antibodies

MYH6/A Alpha-MHC Rabbit polyclonal antibody

Product Sizes
20 ul
£152.00
A12964-20UL
100 ul
£336.00
A12964-100UL
500 ul
£1258.00
A12964-500UL
1000 ul
£2228.00
A12964-1000UL
About this Product
SKU:
A12964
Additional Names:
alpha-MHC|ASD3|CMD1EE|CMH14|MYH6/A Alpha-MHC|MYHC|MYHCA|SSS3
Application:
ELISA, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
224 kDa
Purification:
Affinity Purified
Reactivities:
Mouse, Rat
Sequence:
DPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGV
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A12964
Western blot analysis of various lysates using MYH6/A Alpha-MHC Rabbit pAb (A12964) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded mouse heart using MYH6/A Alpha-MHC Rabbit pAb (A12964) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Immunofluorescence analysis of paraffin-embedded rat heart using MYH6/A Alpha-MHC Rabbit pAb (A12964) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Immunofluorescence analysis of paraffin-embedded mouse heart using MYH6/A Alpha-MHC Rabbit pAb (A12964) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Immunofluorescence analysis of paraffin-embedded rat heart using MYH6/A Alpha-MHC Rabbit pAb (A12964) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.