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Antibodies

[KO Validated] CD133 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A12711-20UL
100 ul
£342.00
A12711-100UL
About this Product
SKU:
A12711
Additional Names:
33|AC133|CD133|CORD12|MCDR2|MSTP061|PROML1|RP41|STGD4
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
97kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
ANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSY
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C 50% glycerol. Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A12711
Western blot analysis of lysates from HCT116 cells, using [KO Validated] CD133 Rabbit pAb (A12711) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Western blot analysis of lysates from wild type (WT) and CD133 knockout (KO) HCT116 cells, using [KO Validated] CD133 Rabbit pAb (A12711) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Immunoprecipitation analysis of 200 ug extracts of HT-29 cells using 3 ug CD133 antibody (A12711). Western blot was performed from the immunoprecipitate using CD133 antibody (A12711) at a dilution of 1:1000.