Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Sonic Hedgehog (Shh) Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A12695-20UL
100 ul
£342.00
A12695-100UL
About this Product
SKU:
A12695
Additional Names:
HHG1|HLP3|HPE3|MCOPCB5|ShhNC|SMMCI|Sonic Hedgehog (Shh)|TPT|TPTPS
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
50kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
PGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDDGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSATGEPEASSGSGPPSGGALG
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A12695
Western blot analysis of various lysates using Sonic Hedgehog (Shh) Rabbit mAb (A12695) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Western blot analysis of various lysates using Sonic Hedgehog (Shh) Rabbit mAb (A12695) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Confocal imaging of paraffin-embedded Mouse kidney using Sonic Hedgehog (Shh) Rabbit mAb (A12695, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.