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Antibodies

[KO Validated] CD44 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A12410-20UL
100 ul
£342.00
A12410-100UL
About this Product
SKU:
A12410
Additional Names:
44|CDW44|CSPG8|ECM-III|ECMR-III|H-CAM|HCELL|Hermes-1|HUTCH-1|HUTCH-I|IN|LHR|MC56|MDU2|MDU3|MIC4|Pgp1
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
82kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
AQIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDV
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A12410
Western blot analysis of various lysates using [KO Validated] CD44 Rabbit pAb (A12410) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from wild type (WT) and CD44 knockout (KO) HeLa cells, using [KO Validated] CD44 Rabbit pAb (A12410) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.