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Antibodies

B Beta-Tubulin Rabbit monoclonal antibody

Product Sizes
50 ul
£146.00
A12289-50UL
100 ul
£205.00
A12289-100UL
200 ul
£318.00
A12289-200UL
500 ul
£640.00
A12289-500UL
1000 ul
£1151.00
A12289-1000UL
About this Product
SKU:
A12289
Additional Names:
CDCBM6|CSCSC1|M40|OK/SW-cl.56|TUBB1|TUBB5|B Beta-Tubulin
Application:
ELISA, IHC-Paraffin, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
50 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
LRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A12289
Western blot analysis of various lysates using B Beta-Tubulin Rabbit mAb (A12289) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
Western blot analysis of lysates from 293F cells, using B Beta-Tubulin Rabbit mAb (A12289) at 1:1000-1:30000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using B Beta-Tubulin Rabbit mAb (A12289) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug B Beta-Tubulin Rabbit mAb (A12289). Western blot was performed from the immunoprecipitate using B Beta-Tubulin Rabbit mAb (A12289) at a dilution of 1:1000.