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Antibodies

[KO Validated] SHMT2 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A1215-20UL
100 ul
£342.00
A1215-100UL
About this Product
SKU:
A1215
Additional Names:
GLYA|HEL-S-51e|mSHMT|NEDCASB|SHMT|T2
Application:
ELISA, IHC-Paraffin, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
56kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
PSPFKHADIVTTTTHKTLRGARSGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLKNARAMADALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKTAKLQDFKSFLLKDSETSQRLANLRQRVEQFARAFPMPGFDEH
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A1215
Western blot analysis of various lysates using SHMT2 Rabbit pAb (A1215) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Western blot analysis of lysates from wild type (WT) and SHMT2 knockout (KO) HeLa cells using [KO Validated] SHMT2 Rabbit pAb (A1215) at 1:400 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 30 s.