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Antibodies

MMP3 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A11418-20UL
100 ul
£342.00
A11418-100UL
500 ul
£1532.00
A11418-500UL
1000 ul
£2704.00
A11418-1000UL
About this Product
SKU:
A11418
Additional Names:
CHDS6|MMP-3|MMP3|SL-1|STMY|STMY1|STR1
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
54 kda
Purification:
Affinity Purified
Reactivities:
Human, Rat
Sequence:
VRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A11418
Western blot analysis of lysates from U-2 OS cells using MMP3 Rabbit mAb (A11418) at 1:1000 dilution incubated overnight at 4°C. U-2 OS cells were treated with IL-1B Beta (20 ng/mL) ,TNF-A Alpha (20 ng/mL) and BFA (300 ng/mL) for 4 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using MMP3 Rabbit mAb (A11418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using MMP3 Rabbit mAb (A11418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of A549 cells using MMP3 Rabbit mAb (A11418, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.