Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

HSP27/HSPB1 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A11156-20UL
100 ul
£342.00
A11156-100UL
500 ul
£1532.00
A11156-500UL
1000 ul
£2704.00
A11156-1000UL
About this Product
SKU:
A11156
Additional Names:
CMT2F|HEL-S-102|HMN2B|HS.76067|Hsp25|HSP27|HSP27/HSPB1|HSP28|SRP27
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
23kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
DVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A11156
Western blot analysis of various lysates using HSP27/HSPB1Rabbit mAb (A11156) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Western blot analysis of various lysates using HSP27/HSPB1Rabbit mAb (A11156) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Confocal imaging of U-2 OS cells using HSP27/HSPB1 Rabbit mAb (A11156, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.