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Antibodies

FAK Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A11131-20UL
100 ul
£342.00
A11131-100UL
500 ul
£1532.00
A11131-500UL
1000 ul
£2704.00
A11131-1000UL
About this Product
SKU:
A11131
Additional Names:
FADK|FADK 1|FAK|FAK1|FRNK|p125FAK|pp125FAK|PPP1R71
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
119kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
TVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIG
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A11131
Western blot analysis of various lysates using FAK Rabbit mAb (A11131) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from MCF7 cells, using FAK Rabbit mAb (A11131) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Confocal imaging of HeLa cells using FAK Rabbit mAb (A11131,at dilution of 1:100) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.