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Antibodies

Cyclin D1 Rabbit polyclonal antibody

Product Sizes
20 ul
£152.00
A11022-20UL
100 ul
£336.00
A11022-100UL
500 ul
£1258.00
A11022-500UL
1000 ul
£2228.00
A11022-1000UL
About this Product
SKU:
A11022
Additional Names:
BCL1|Cyclin D1|D11S287E|PRAD1|U21B31
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
34kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
PSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A11022
Western blot analysis of lysates from Hep G2 cells using Cyclin D1 Rabbit pAb (A11022) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30 s.
Western blot analysis of various lysates using Cyclin D1 Rabbit pAb (A11022) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60 s.
Immunohistochemistry analysis of paraffin-embedded Human colon using Cyclin D1 Rabbit pAb (A11022) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using Cyclin D1 Rabbit pAb (A11022) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.