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Antibodies

CBL Rabbit polyclonal antibody

Product Sizes
20 ul
£152.00
A0732-20UL
100 ul
£336.00
A0732-100UL
500 ul
£1258.00
A0732-500UL
1000 ul
£2228.00
A0732-1000UL
About this Product
SKU:
A0732
Additional Names:
C-CBL|CBL|CBL2|FRA11B|NSLL|RNF55
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
100kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
AATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A0732
Western blot analysis of lysates from 293T cells, using CBL Rabbit pAb (A0732) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Western blot analysis of lysates from Mouse thymus, using CBL Rabbit pAb (A0732) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human prostate using CBL Rabbit pAb (A0732) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using CBL Rabbit pAb (A0732) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of K-562 cells using CBL Rabbit pAb (A0732) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.