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Antibodies

Histone H1.2 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A0646-20UL
100 ul
£342.00
A0646-100UL
About this Product
SKU:
A0646
Additional Names:
H1.2|H1C|H1F2|H1s-1|HIST1H1C|Histone H1.2
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
21kDa
Purification:
Affinity Purified
Reactivities:
Human, Other Species
Sequence:
MSETAPAAPAAAPPAEKAPVKKKAAKKAGGTPRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTG
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A0646
Western blot analysis of various lysates using Histone H1.2 Rabbit mAb (A0646) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Confocal imaging of U-2 OS cells using Histone H1.2 Rabbit mAb (A0646,dilution 1:100)(Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.