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Antibodies

Neurofilament L Rabbit polyclonal antibody

Product Sizes
20 ul
£152.00
A0257-20UL
100 ul
£336.00
A0257-100UL
500 ul
£1258.00
A0257-500UL
1000 ul
£2228.00
A0257-1000UL
About this Product
SKU:
A0257
Additional Names:
CMT1F|CMT2E|CMTDIG|Neurofilament L|NF-L|NF68|NFL|PPP1R110
Application:
ELISA, IHC-Paraffin, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
62 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
SSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVT
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A0257
Western blot analysis of various lysates using Neurofilament L Rabbit pAb (A0257) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Neurofilament L Rabbit pAb (A0257) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using Neurofilament L Rabbit pAb (A0257) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Neurofilament L Rabbit pAb (A0257) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded rat brain using Neurofilament L Rabbit pAb (A0257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.