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Antibodies

[KO Validated] Bax Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A0207-20UL
100 ul
£342.00
A0207-100UL
About this Product
SKU:
A0207
Additional Names:
BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4,BAX
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
21 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A0207
Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells using [KO Validated] Bax Rabbit pAb (A0207) at 1:3000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5 s.
Western blot analysis of various lysates using [KO Validated] Bax Rabbit pAb (A0207) at 1:3000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5 s.
Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.