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Antibodies

CaMK2 delta/CAMK2 gamma Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A0186-20UL
100 ul
£342.00
A0186-100UL
About this Product
SKU:
A0186
Additional Names:
CAM2|CAMK2|CaMK2 delta/CAMK2 gamma|CAMKB|CaMKIIbeta|MRD54
Application:
ELISA, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
73 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
CGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A0186
Western blot analysis of various lysates using CaMK2 delta/CAMK2 gamma Rabbit mAb (A0186) at 1:11000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunoprecipitation of CaMK2 delta/CAMK2 gamma from 300 ug extracts of 293T cells was performed using 2 ug of CaMK2 delta/CAMK2 gamma Rabbit mAb (A0186). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using CaMK2 delta/CAMK2 gamma Rabbit mAb (A0186) at a dilution of 1:10000.