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Antibodies

ALDH1A1 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A0157-20UL
100 ul
£342.00
A0157-100UL
About this Product
SKU:
A0157
Additional Names:
ALDC|ALDH-E1|ALDH1|ALDH11|ALDH1A1|HEL-9|HEL-S-53e|HEL12|PUMB1|RALDH1
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human ALDH1A1 (NP_000680.2).
Isotype:
IgG
Molecular Weight:
55kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
VGKLIKEAAGKSNLKRVTLELGGKSPCIVLADADLDNAVEFAHHGVFYHQGQCCIAASRIFVEESIYDEFVRRSVERAKKYILGNPLTPGVTQGPQIDKEQ
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A0157
Western blot analysis of various lysates, using ALDH1A1 Rabbit mAb (A0157) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): THP-1 Exposure time: 10s.
Western blot analysis of various lysates, using ALDH1A1 Rabbit mAb (A0157) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Confocal imaging of Hep G2 cells using ALDH1A1 Rabbit mAb (A0157, dilution 1:8000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of A549 cells using ALDH1A1 Rabbit mAb (A0157, dilution 1:8000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation analysis of 300 ug extracts of A-549 cells using 3 ug ALDH1A1 Rabbit mAb antibody (A0157). Western blot was performed from the immunoprecipitate using ALDH1A1 Rabbit mAb (A0157) at a dilition of 1:20000.