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ImmPACT[R] VIP Substrate, Peroxidase (HRP)

Product Sizes
About this Product
Additional Names:
Enzyme Substrates [Enzyme Substrates ] Precipitating HRP Substrate, Horseradish Peroxidase substrate
Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, ELISPOT, FLUOROSPOT
Extra Details:
Vector[R] VIP and ImmPACT[TM] VIP HRP substrates both produce intense, violet (purple) reaction products, and can be used as alternatives to DAB or as a second color for multiple antigen labeling. Vector[R] VIP and ImmPACT[TM] VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector[R] VIP and ImmPACT[TM] VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.

This product utilizes Vector Laboratories novel ImmPACT[TM] technology to generate an intense violet reaction product that is 2-4 times more sensitive than Vector [R]VIP substrate kit (SK-4600). This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT[TM] VIP reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4 °C.


  • 2-4 fold greater sensitivity than original substrate kit
  • Optimized for IHC/ICC/ISH and blots
  • Permanent mounting
  • Ideal for single and multiple labeling
  • Working solutions stable for up to 14 days at 4 °C
  • One year expiry date
  • Supplied as 120 ml of diluent and stock solutions in dropper bottles
  • Shipping Conditions:
    Storage Conditions:
    2-8 [o]C
    Vector Laboratories