ClCN3 Antibody, Clone S258-5: ATTO 390 SKU: SMC-408D-A390 Additional Names: CIC3 Antibody, CLC3 Antibody, CLCN 3 Antibody, Chloride channel 3 Antibody, Chloride channel protein 3 Antibody, Chloride channel Antibody, voltage-sensitive 3 Antibody, Chloride transporter ClC 3 Antibody, DKFZp564I0463 Antibody, H(+)/Cl(-) exchange transporter 3 Antibody, Chloride transporter ClC-3 Antibody, ClC-3 Antibody, H(+)/Cl(-) exchange transporter 3 Antibody Application: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot CE/IVD: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. Clone: S258-5 Clonality: Monoclonal Conjugate: ATTO 390 Concentration: 1 mg/ml Extra Details: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory. Gene Details: Clcn3 Host: Mouse Immunogen: Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3 Isotype: IgG1 Protein Details: P51792 Purification: Protein G Purified Reactivities: Human, Mouse, Rat Shipping Conditions: Blue Ice Storage Conditions: -20[o]C Supplier: StressMarq Biosciences Type: Antibody: Monoclonal Antibody Size: 100 ug Specificity: Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results). Preservative: 0.09% Sodium azide Long Description: Mouse Anti-Rat Clcn3 Monoclonal IgG1 Accession: NP_445815.2 Synonyms: chloride channel 3;chloride channel protein 3;chloride channel, voltage-sensitive 3;chloride transporter ClC-3;ClC-3;H(+)/Cl(-) exchange transporter 3;protein kinase C-regulated chloride channel Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Clcn3 Monoclonal Antibody, Clone S258-5 (SMC-408). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody (SMC-408) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Clcn3 Antibody. (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5 (SMC-408). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody (SMC-408) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa. Related Products VECTASHIELD[R] PLUS Antifade Mounting Medium with DAPI Price for 10ml £214.00 View Product VECTASHIELD[R] PLUS Antifade Mounting Medium with DAPI Price for 2ml £62.00 View Product VECTASHIELD[R] PLUS Antifade Mounting Medium Price for 10ml £198.00 View Product Price for 100 ug £460.00 Qty: Add to order Hi, 2B Team here, how can we make a difference? Chat Academic Pricing Please contact us for academic pricing. Contact Us Related Products VECTASHIELD[R] PLUS Antifade Mounting Medium with DAPI Price for 10ml £214.00 View Product VECTASHIELD[R] PLUS Antifade Mounting Medium with DAPI Price for 2ml £62.00 View Product VECTASHIELD[R] PLUS Antifade Mounting Medium Price for 10ml £198.00 View Product