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10X RIPA Lysis Buffer

Product Sizes
15 ml
MB-077-0015-15ML
About this Product
SKU:
MB-077-0015
Additional Names:
10X RIPA Lysis Buffer, RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer
Application:
ChIP, Immunoprecipitation, Western Blot
Buffer:
See application note.
CE/IVD:
RUO
Concentration:
10X
Extra Details:
RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash buffer for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of this buffer. In addition, RIPA Lysis Buffer minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. The following RIPA Lysis Buffer components have the following effects: Tris-HCl is a buffering agent prevents protein denaturation, NaCl is a salt that prevents non-specific protein aggregation, IGEPAL is a non-ionic detergent to extract proteins; Na-deoxycholate and SDS are ionic detergents to extract proteins; and sodium azide is a bacteriostatic agent added to retard bacterial growth. RIPA Lysis Buffer is supplied as a ready-to-use solution that requires no preparation. We suggest that the user add protease and phosphatase inhibitors not included with this product prior to use.
Physical State:
Liquid
Shipping Conditions:
Ambient
Specificity:
This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Buffers & General Consumables: Others
Comparison of miR-34a and target oncogene expression levels in 143B cells treated with bioengineered miR-34a prodrug (tRNA/mir-34a) and doxorubicin, alone or in combination. Cells were harvested at 72 h after treatment. Pre-miR-34a. (C) were measured by Western blot. Band density was determined by Image Lab software (Bio-Rad), and normalized to that of GAPDH. Cell lysates were prepared using RIPA buffer (p/n MB-077) supplemented with the complete protease inhibitor cocktail, and protein concentrations were determined using a BCA Protein Assay Kit. Figure 7. PMID: 26518752.
10X RIPA Lysis Buffer
Representative picture above of Western blots from LV-free wall tissues in each group (control group, 10; MI group, 5; LL-TS group, 5) showed effects of LL-TS treatment on protein expression level of TGF-B Beta1, MMP-9, collagen I, and collagen III. Transmural myocardial tissue sample ~1 cm2 obtained from the LV free wall outside the infarction area was homogenized in radioimmunoprecipitation assay lysis buffer (p/n MB-077) containing proteinase inhibitor. Fig 5. PMID: 25332149.