BlockOut[R] Universal Blocking Buffer for Western Blotting
Product Sizes
500 ml
MB-073-500ML
About this Product
- SKU:
- MB-073
- Additional Names:
- Multiplex Blocking Buffer, Immunoblot Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer, colorimetric, chemiluminescent blocking buffer, WB block, BlockOut, Block out
- Application:
- Western Blot
- Buffer:
- See application note.
- CE/IVD:
- RUO
- Concentration:
- 1X
- Extra Details:
- BlockOut® is an universal blocking buffer solution for Western Blotting. It is specifically designed for colorimetric, chemiluminescent, and fluorescent western blotting. Universal Blocking Buffer is recommended for blocking when phospho specific antibodies are used. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with chemiluminescent and fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
- Physical State:
- Liquid
- Shipping Conditions:
- Blue Ice
- Specificity:
- BlockOut® Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
- Storage Conditions:
- 2-8[o]C Do not freeze.
- Supplier:
- Rockland Inc
- Type:
- Buffers & General Consumables: Others
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=MB-073
