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Antibodies

GFP Antibody

Product Sizes
25 ul
600-901-215S-25UL
About this Product
SKU:
600-901-215S
Additional Names:
chicken anti-GFP antibody, chicken Anti-Green Fluorescent Protein Antibody, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish antibody
Application:
Detection/Quantitation, Dot Blot, Electron Microscopy, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Purification, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.17 mg/ml
Extra Details:
Chicken Anti-GFP is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. GFP Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Chicken IgY lacks the classic "Fc" domain and does not bind to mammalian IgG Fc receptors. Thus resulting in lower backgrounds for western blotting, ELISA and Immunohistochemistry.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Chicken/Avian
Immunogen:
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Isotype:
IgY
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Dry Ice
Specificity:
Anti-GFP Antibody IgY purification was prepared from egg yolks by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Prot
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot Results of Chicken Anti-GFP Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: GFP (p/n 000-001-215) / HeLa WCL (p/n W09-000-364) [0.05ug/10ug]. Lane 3: GFP (p/n 000-001-215) / NIH3T3 WCL (p/n W10-000-371) [0.05ug/10ug]. Lane 4: GFP (p/n 000-001-215) / PC-12 WCL (p/n W120-001-GL9) [0.05ug/10ug]. Lane 5: HeLa WCL (p/n W09-000-364) [10ug]. Lane 6: NIH3T3 WCL (p/n W10-000-371) [10ug]. Lane 7: PC-12 WCL (p/n W120-001-GL9) [10ug]. Primary Antibody: Anti-GFP at 1.0ug/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Chicken IgG HRP (p/n 603-103-126) at 1:40,000 for 30 mins at RT. Block: MB-070 Buffer for 1hr at RT. Predicted MW: 27kda. Exposure: 2 seconds.
Western Blot of Chicken anti-GFP Antibody with Serums. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: GFP/Human Serum (p/n 000-001-215/D314-05) [0.01/0.02uL] [+]. Lane 3: Human Serum (p/n D314-05) [0.02uL] [-]. Lane 4: GFP/Mouse Serum (p/n 000-001-215/D308-05) [0.01/0.02uL] [+]. Lane 5: Mouse Serum (p/n D308-05) [0.02uL] [-]. Lane 6: GFP/Rat Serum (p/n 000-001-215/D310-05) [0.01/0.02uL] [+]. Lane 7: Rat Serum (p/n D310-05) [0.02uL] [-]. Primary antibody: Anti-GFP antibody at 1.0ug/mL overnight at 2-8°C. Secondary antibody: Donkey Anti-Chicken IgG HRP secondary antibody (p/n 603-103-126) at 1:40,000 for 30 min at RT. Blocking Buffer: BlockOut Buffer (p/n MB-073) for 1hr at RT. Predicted/Observed size: ~27kDa for GFP.
Immunofluorescence Microscopy of chicken anti-GFP antibody. Tissue: KruppleGAL4 driver line in Drosophila eye disc. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: anti-GFP antibody diluted 1:500 for 2 hr at RT. Secondary antibody: Alexa™488 conjugated anti-Chicken IgG at 1:300 for 1 hr at RT. Blocking: 5% NGS in PBS with 0.1% Triton X-100 for 15 min. Staining: recombinant tau-myc-GFP protein as green fluorescent signal.
Western Blot of anti-GFP antibody. Lane 1: mouse spleen lysate (p/n W10-000-T029). Lane 2: mouse spleen lysate spiked with 50 ng of wt GFP (p/n W10-000-T029/000-001-215). Load: 20 ug per lane. Primary antibody: GFP antibody at 2 ug/ml for 2 hr at room temperature. Secondary antibody: IRDye™800 Conjugated Affinity Purified anti-Chicken IgG [H&L] [Goat] MX10 (p/n 603-132-126) at 1:20,000 for 45 min at RT. Block: 5% BSA in PBS 2 hr at room temperature. Predicted/Observed size: 27 kDa for GFP epitope tag. Other band(s): none.
Western Blot of Anti-GFP (CHICKEN) antibody. Lane 1: MW. Lane 2: GFP (p/n 000-001-215). Load: 0.05 ug. Primary antibody: Anti-GFP (CHICKEN) antibody (p/n 600-901-215) was used at 1:1000 overnight at 4°C. Secondary antibody: Anti-Chicken IgG (GOAT) peroxidase conjugated antibody (p/n 603-103-126) secondary antibody was used at 1:40,000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). Block: 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.