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Antibodies

IKK E phospho T501 Antibody

Product Sizes
100 ug
600-401-267-100UG
About this Product
SKU:
600-401-267
Additional Names:
IKBKE|rabbit anti-IKK epsilon pT501 antibody, rabbit anti-IKKE pT501 antibody, I kappa B kinase epsilon antibody, IKK-epsilon, IKK-E, Inducible I kappa-B kinase, IkBKE antibody, IKBKE protein antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKepsilon is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFA Alpha or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa. Anti-IKKE Epsilon pT501 antibody is ideal for investigators involved in NFkappaB and apoptosis research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
IKKe phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-IKKE Epsilon pT501 antibody was affinity purified from monospecific antiserum by immunoaffinity purification against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity. This phospho specific polyclonal antibody is specific for phosphorylated pT50
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
IFI44 decreases the kinase activity of IKKB Beta and IKKE Epsilon. Human 293T cells were silenced for IFI44, or for FKBP5, and were transfected with plasmids expressing His-IKKE Epsilon (A) or MYC-IKKB Beta (B), together with IFI44-HA, and FKBP5-FLAG expression plasmids. At 24 hpt, IKKE Epsilon (A) and IKKB Beta (B) complexes were purified with anti-His and anti-MYC antibodies, respectively, and these complexes were assayed in kinase assays using IRF-3 (for the IKKE Epsilon complexes shown in panel A) and IkBA Alpha (for the IKKB Beta complexes shown in panel B) as substrates. The levels of phosphorylated and unphosphorylated forms of IRF-3 (panel A, bottom blot) and IkBA Alpha (panel B, third and fourth blots) were analyzed by Western blotting using specific antibodies. Levels of IKKE Epsilon were analyzed using an anti-His-specific antibody (A, first blot) and anti-pIKKE Epsilon (A, second blot), and levels of IKKB Beta were analyzed using an anti-MYC-specific antibody (B, first blot) and anti-pIKKB Beta (B, second blot). Western blots were quantified by densitometry using ImageJ software (v1.46). Protein expression levels in cells expressing IKKE Epsilon (A) and IKKB Beta (B) alone were assigned a value of 100% for comparisons with the levels of expression in cells expressing the different combinations of IKKE Epsilon/IFI44/FKBP5 (A) or IKKB Beta/IFI44/FKBP5 (B) (numbers are indicated below each plot). pIRF-3 and IRF-3 levels (observed in the same bottom blot in panel A) and pIkBA Alpha and IkBA Alpha (third and bottom blot in panel B) are represented with numbers below each blot. Levels of pIRF-3 and pIkBA Alpha normalized to the levels of IKKE Epsilon and IKKB Beta are represented in the bottom graphs in panels A and B, respectively. Molecular weight markers are indicated (in kilodaltons) on the right. Figure provided by CiteAb. Source: MBio, PMID: 31455651.