Avidin Antibody Biotin Conjugated
Product Sizes
100 ug
200-4697-0100-100UG
About this Product
- SKU:
- 200-4697-0100
- Additional Names:
- rabbit anti-Avidin Antibody biotin Conjugation, Biotin conjugated rabbit anti-Avidin antibody, Avidin BAC, Anti-Avidin biotin Antibody, Egg White
- Application:
- ELISA, FISH, Immunofluorescence, Immunohistochemistry, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- Clonality:
- Polyclonal
- Conjugate:
- Biotin
- Concentration:
- 1 mg/ml
- Extra Details:
- Avidin is a glycoprotein with a molecular weight of approximately 62.4 kDa. Avidin is a biotin binding protein that shows high sequence homology in birds, reptiles and amphibians. Hen egg white avidin is a tetrameric protein composed of four identical subunits, each with the ability to bind biotin with high affinity and specificity (Kd ~ 1015 M). In biotechnology, the functional consequence of tetrameric biotin binding is signal amplification. Biotin-avidin bridging is a great way to increase signal strength while maintaining specificity. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Rabbit
- Immunogen:
- Avidin (Hen Egg White)
- Isotype:
- IgG
- Physical State:
- Lyophilized
- Reactivities:
- Label/Epitope-tag
- Shipping Conditions:
- Ambient
- Specificity:
- Anti-Avidin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, as well as purified a
- Storage Conditions:
- 2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Polyclonal Antibody
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=200-4697-0100
