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Human IFN-gamma/IL-10 FluoroSpotPLUS kit

SKU:
FSP-0107-2
Academic Pricing:
Please contact us at info@2bscientific.com for specific academic pricing
Additional Names:
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN--gamma/IL-10 FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (monoclonal antibody (mAb) clone CD3-2) is supplied as a positive control for cytokine secretion.
Application:
FluoroSpot
Format:
pre-coated plates
Gene Details:
3458, 3586
Immunogen:
IFNG, IL10
Reactivities:
Human
References:
Jackson, S. E., et al. (2017). Latent Cytomegalovirus (CMV) Infection Does Not Detrimentally Alter T Cell Responses in the Healthy Old, But Increased Latent CMV Carriage Is Related to Expanded CMV-Specific T Cells. Front Immunol. (http://www.ncbi.nlm.nih.gov/pubmed/28694811) Liebowitz, D., et al. (2020). Efficacy, immunogenicity, and safety of an oral influenza vaccine: a placebo-controlled and active-controlled phase 2 human challenge study. Lancet Infect Dis. (http://www.ncbi.nlm.nih.gov/pubmed/31978354) Kaiser, Y., et al. (2016). Expanded lung T-bet+RORgammaT+ CD4+ T-cells in sarcoidosis patients with a favourable disease phenotype. Eur Respir J. (http://www.ncbi.nlm.nih.gov/pubmed/27230441) Mateus, J., et al. (2020). Selective and cross-reactive SARS-CoV-2 T cell epitopes in unexposed humans. Science. (http://www.ncbi.nlm.nih.gov/pubmed/32753554) Danielzik, T., et al. (2017). Sensitive detection of rare antigen-specific T cells directed against Wilms' tumor 1 by FluoroSpot assay. Leuk Lymphoma. (http://www.ncbi.nlm.nih.gov/pubmed/28573960)
Shipping Conditions:
Ambient
Supplier:
Mabtech
Related Product 1:
FSP-010703-2
Price £706.00

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