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Human IFN-gamma/IL-10 FluoroSpotPLUS kit

SKU:
FSP-0107-10
Academic Pricing:
Please contact us at info@2bscientific.com for specific academic pricing
Additional Names:
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN--gamma/IL-10 FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (monoclonal antibody (mAb) clone CD3-2) is supplied as a positive control for cytokine secretion.
Application:
FluoroSpot
Format:
pre-coated plates
Gene Details:
3458, 3586
Immunogen:
IFNG, IL10
Reactivities:
Human
References:
Jackson, S. E., et al. (2017). HCMV Specific CD4+ T Cells are poly-functional and can respond to HCMV Infected Dendritic Cells in vitro. Journal of virology. (http://www.ncbi.nlm.nih.gov/pubmed/28053099) Weinberg, A., et al. (2015). Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. PLoS ONE. (http://www.ncbi.nlm.nih.gov/pubmed/25874544) Mateus, J., et al. (2020). Selective and cross-reactive SARS-CoV-2 T cell epitopes in unexposed humans. Science. (http://www.ncbi.nlm.nih.gov/pubmed/32753554) Kaiser, Y., et al. (2016). Expanded lung T-bet+RORgammaT+ CD4+ T-cells in sarcoidosis patients with a favourable disease phenotype. Eur Respir J. (http://www.ncbi.nlm.nih.gov/pubmed/27230441) Lee, F. E., et al. (2007). Human infant respiratory syncytial virus (RSV)-specific type 1 and 2 cytokine responses ex vivo during primary RSV infection. The Journal of infectious diseases. (http://www.ncbi.nlm.nih.gov/pubmed/17492594)
Shipping Conditions:
Ambient
Supplier:
Mabtech
Related Product 1:
FSP-010703-2
Price £3047.00

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