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Assay Kits & ELISAs

Cell-mediated Cytotoxicity Assay: Basic Cytotoxicity Test

Product Sizes
125 tests
969-125TESTS
About this Product
SKU:
969
Application:
Flow Cytometry
Extra Details:
A dual-color assay for determining cell toxicity by flow cytometry. A green fluorescent cellular stain, CFSE, labels target cells and the red live/dead viability dye 7-AAD to identify the dead cells present in the cytotoxicity assay samples.
Immunogen:
Cell-mediated cytotoxicity
Physical State:
Cell Culture
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Quantification of cell death
Figure 10. Control F identifies green target cells. See protocol for details.
Figure 11. Calculation of cytotoxicity. Based on the number of green target cells in R3 (Figures 10 and 12) cytotoxicity can be calculated as the percentage of the green target cells which are also stained red with 7-AAD in R2 (Figure 10). This analysis reveals the population of necrotic target cells without interference from necrotic effector cells. Our Basic Cytotoxicity Assay is the easiest test to quantify cytotoxicity.
Figure 12. An optimal E:T cell ratio is required to effectively determine cytolytic activity. Determine this by running an experiment at several different ratios. For example: K562 target cells were stained with CFSE and adjusted to 1.5 x 104 cells/tube. Effector cells were added at a ratio of 0:1, 12.5:1, 25:1, 50:1, or 100:1 and incubated for 4 hours to allow the cytolytic activity to progress. 7-AAD was then added to label necrotic cells red. See protocol for details.
Figure 2. Assay procedure