Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Assay Kits & ELISAs

Green Fluorescent FAM-Leu-CMK Serine Protease Assay Kit

Product Sizes
25 tests
949-25TESTS
About this Product
SKU:
949
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
Quantify intracellular active chymotrypsin-like serine protease activity in vitro. The CMK group alkylates the active site histidine residue. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader., ImmunoChemistry Technologies
Immunogen:
Chymotrypsin-like enzymes
Physical State:
Cell Culture, Tissue
Purification:
Intracellular serine protease activity was detected in Jurkat cells using ICT's green FAM-FLISP serine protease inhibitor FLCK (Kit #950) and cells with compromised membrane activity were identified u
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C, -20[o]C
Type:
Assay Kits: Detection
Figure 1. Jurkat cells were untreated (A.), or were exposed to staurosporine to induce apoptosis and increase serine protease activity (B.). During the final hour of treatment, samples were stained with FAM-Leu-CMK (Cat. 949/950) for 1 hour at 37°C, washed, stained with PI for 15 minutes, and then analyzed using a flow cytometer. Staurosporine increased median fluorescence intensity for both FAM-Leu-CMK (BL1-H, x-axis) and PI (YL1-H, y-axis). Data courtesy of Dr. K. Strandberg (ICT 231:80-81).