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Assay Kits & ELISAs

Green Fluorescent FAM-Leu-CMK Serine Protease Assay Kit

Product Sizes
25 tests
949-25TESTS
About this Product
SKU:
949
Application:
Flow Cytometry
Extra Details:
Quantify intracellular active chymotrypsin-like serine protease activity in vitro. The CMK group alkylates the active site histidine residue. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
Immunogen:
Chymotrypsin-like enzymes
Physical State:
Cell Culture, Tissue
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Detection
Figure 1. Jurkat cells were untreated (A.), or were exposed to staurosporine to induce apoptosis and increase serine protease activity (B.). During the final hour of treatment, samples were stained with FAM-Leu-CMK (Cat. 949/950) for 1 hour at 37°C, washed, stained with PI for 15 minutes, and then analyzed using a flow cytometer. Staurosporine increased median fluorescence intensity for both FAM-Leu-CMK (BL1-H, x-axis) and PI (YL1-H, y-axis). Data courtesy of Dr. K. Strandberg (ICT 231:80-81).