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Assay Kits & ELISAs

Magic Red[R] Fluorescent Caspase-3/7 Assay Kit

Product Sizes
25 tests
935-25TESTS
About this Product
SKU:
935
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
The Magic Red® reagent fluoresces upon cleavage by active caspase 3 and caspase 7 enzymes, enabling real-time detection of activity. Analyze the fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or flow cytometry., ImmunoChemistry Technologies
Immunogen:
Caspase-3/7
Physical State:
Cell Culture, Tissue
Purification:
Rat fibroblasts were seeded in a 12-well plate at 1x104 cells/mL and irradiated the following day. 23.1 uL of MR-(DEVD)2 solution was mixed with media and added. 1 hour later, 300 uL media was added a
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Cresyl Violet Perchlorate Excitation and Emission Spectra in Ethanol
Figure 10. Cells were incubated with 1 uM staurosporine (cat. 6212) or DMSO control for 3 hours (Jurkat cells) or 4 hours (HL-60, U937, THP-1 cells) at 37°C. Cells were incubated with MR-(DEVD)2 for 1-2 hours to measure intracellular DEVDase activity. Activity is shown after 1 hour (solid bars) and 2 hour (spotted bars) exposure to MR-(DEVD)2. Analyzed using a fluorometric plate reader with 590 nm excitation, 640 nm emission, with a 630 nm cut-off filter. Data courtesy of Dr. Brian Lee, ICT.
Figure 11. Jurkat cells were treated with a negative control (left) or staurosporine (right), for 4 hours, then labeled with MR-(DEVD)2 (cat. 935/936) for 1 hour. Cells were analyzed in a flow cytometer using excitation at 640 nm and a 675/25 emission filter pairing (FL4). Only 4.2% of control cells were apoptotic (left, V2-R), whereas treatment with staurosporine induced activity in 93.2% of cells (right, V2-R). This is a ratio of 22:1. Data courtesy of Ms. Tracy Hanson, ICT, 082313.
Figure 12. Jurkat cells were treated with a negative control (left) or staurosporine (right), then labeled with MR-(DEVD)2 (cat. 935), followed by Green Live/ Dead Stain (cat. 6342). Only 2.9% of control cells were labeled (UR + LR, left plot), whereas 81.6% of the induced cells were labeled (UR + LR, right plot). Staurosporine increased the proportion of necrotic or late stage apoptotic cells from 5.9% (UL + UR, left) to 35.6% (UL + UR, right). Data courtesy of Ms. Tracy Hanson, ICT, 092614.
Figure 2. MCF-7 cells were exposed to 0.15 uM camptothecin (cat. 6210) for 24 hours at 37°C, then stained with MR-(DEVD)2 for 30 minutes at 37°C, washed, and then stained with 1 ug/mL Hoechst stain. Apoptotic cells with orange-red lysosomal bodies and less intense blue nuclei are intermixed with non-apoptotic cells bearing bright blue nuclei and absent or reduced orange-red lysosomal staining. Photo provided by Dr. Zbigniew Darzynkiewicz at Brander Cancer Research Center Institute, New York, NY.