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Assay Kits & ELISAs

Green Fluorescent FAM-FLICA[R] Caspase-3/7 (DEVD) Assay Kit

Product Sizes
25 tests
93-25TESTS
About this Product
SKU:
93
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
This in vitro assay employs the green fluorescent inhibitor probe FAM-DEVD-FMK to label active caspase 3 and caspase 7 enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry., ImmunoChemistry Technologies
Immunogen:
Caspase-3/7
Physical State:
Cell Culture
Purification:
Normal (left) and keratoconus (right) corneal fibroblasts were treated with 200 uM H2O2 for 1 hr, washed, and allowed to recover for 1-3 hrs. The culture media was removed and replaced with 1X FAM-FLI
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Cell death in primary rat hippocampal neurons. (A) is a composite of FLICA® FAM-DEVD-FMK (B), PI (C), and Hoechst (D). DNA is labeled in 4 cells (D), 3 cells are caspase-positive (B), 2 are membrane-compromised (C). One cell is in early apoptosis (green but not red). Two FLICA®-positive cells are also PI-positive (red, C) (becoming membrane compromised) and are in the late stages of apoptosis rather than necrosis. Data courtesy of Dr. Z. Kahraman Akozer, University of Maryland.
Figure 2. Normal (A) and keratoconus (B) corneal fibroblasts were treated with 200 uM H2O2 for 1 hour, washed, and allowed to recover. Cells were then treated with FAM-DEVD-FMK (cat. 93/94) for 1 hour, then washed with 1X Apoptosis Wash Buffer. Keratoconus corneal fibroblasts treated with H2O2 (B) show a significant increase in caspase-3/7 activity compared to normal cells (A). Non-apoptotic cells are dark in background. Data courtesy of Dr. Cristina Kenney, University of California, Irvine.